
KM-H2
ACC 8
Cell line:
KM-H2
DSMZ no.:
ACC 8
Species:
human (Homo sapiens)
Cell type:
Hodgkin lymphoma
Origin:
established from the pleural effusion of a 37-year-old man with Hodgkin lymphoma (mixed cellularity progressing to lymphocyte depletion; stage IV at relapse) in 1974. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq)
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.8 x 106 cells/ml; viability may drop to about 50%, but cells will recover within a week; then, maintain at 0.3-1.0 x 106 cells/ml; split 1:2 to 1:4 every second day;
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 48 hours
Harvest:
saturation density at about 1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
CD2-, CD3-, CD13-, CD14+, CD15+, CD19-, CD21+, CD25-, CD30+, CD34-, HLA-DR+; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 3.5% polyploidy - 59(56-60)<3n>der(X)der(Y), -X, -1, -1, -2, -2, -3, -4, -5, -5, -6, -8, -9, -9, -10, -10, -10, -11, -12, -13, -14, -15, -15, -16, -16, -18, -19, -20, -21, -22, +25mar - add(1)(q32), add(3)(q26), add(3)(q26), del(4)(q26q31), i(4q), add(5)(p11), add(6)(q14), add(7)(p14), add(7)(q34), del(7)(q31), add(8)(q24), del(8)(p12), del(11)(q23), i(13q), der(13)t(11;13)(p13;q13), add(14)(p12)x2, del(17)(p12), i(18q) - apparently typical HD karyotype with extraordinary level of rearrangements including several HSR-bearing markers with ectopic rDNA - resembles published karyotype
Molec. Genetics:
expression of fusion genes PRRC2B-MGMT (BAT2L1-MGMT) and CIITA-BX648577 confirmed by RT-PCR
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list