GL-261
ACC 802
Cell line:
GL-261
DSMZ no.:
ACC 802
Species:
murine (mouse) (Mus musculus)
Cell type:
glioblastoma
Origin:
established as in vitro growing cell culture in the mid 1990s from the Gl261 tumor which was induced in 1939 by intracranial injection of 3-methylcholanthrene into C57BL/6 mice and subsequently maintained by serial transplantation in syngeneic mouse strain; cells were described in the literature to carry TP53 and KRAS mutations
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS + 2 mM glutamine
Subculture:
seed out at ca. 1 x 106 cells/175 cm2 flask; split 1:3 - 1:4 every 5-6 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 80-100 hours
Harvest:
cell harvest of ca. 6 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list