human (Homo sapiens)
etablished in 2015 from the temporal lobe brain tumor diagnosed as glioblastoma multiforme of a 74-year-old woman
Permissions and restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
90% MEM (with Earl´s salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids
seed out at ca. 0.5-1 x 106 cells/175 cm2 flask; split 1:3 to 1:6 every 3-7 days using trypsin/EDTA
at 37 °C with CO2
ca. 40-60 hours
cell harvest of ca. 5-11 x 106 cells/175 cm2
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay
to inquire about expression of EpCAM and intermediate filaments, contact email@example.com.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
|Frozen culture||400,- €|
|Growing culture |
(please inquire for exact delivery time)
|DNA isolated from cell line (25 µg)||500,- €|
|DNA isolated from cell line (5 µg)||120,- €|
see price list