JVE-059
ACC 806
Cell line:
JVE-059
DSMZ no.:
ACC 806
Species:
human (Homo sapiens)
Cell type:
colon carcinoma
Origin:
established from the tumor mass of a 58-year-old man with colorectal adenocarcinoma of the colon transversum (Duke stage D); cells were described in the literature to exhibit microsatellite instability (MSI-H) and a partial methylation of the CDKN2A promoter
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/25 cm2 flask; split confluent culture 1:2 once or twice a week using trypsin/EDTA (we recommend to wash the cells at least two times with PBS and incubate with trypsin for a minimum of 5 min.); avoid a long thawing procedure to prevent a decrease in viability
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3-4 days
Harvest:
cell harvest of ca. 10 x 106 cells/25 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list