COLO-818
ACC 81
Cell line:
COLO-818
DSMZ no.:
ACC 81
Species:
human (Homo sapiens)
Cell type:
melanoma
Origin:
established from malignant melanoma
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
split confluent culture about 1:6 once a week using trypsin/EDTA; seed out at about 1 x 106 cells/80 cm2
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 60 hours
Harvest:
cell harvest of about 8 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 30% polyploidy - 62(57-63)<3n>X, -X, -X, -4, -5, +7, +8, -9, -10, -11, -13, +15, -16, -17, -19, -21, -21, +3mar, 1 dm present in 15% cells - add(3)(p11), der(13;21)(p10;10) - high level of subclonal rearrangement present - closely related to COLO-800 (DSM ACC 193)
Molec. Genetics:
Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list