GH
ACC 811
Cell line:
GH
DSMZ no.:
ACC 811
Species:
human (Homo sapiens)
Cell type:
testicular germ cell tumor
Origin:
established in 1980 from a human testicular teratocarcinoma of a male patient of unknown age; cells are described in the literature to express mRNA for endogenous retrovirus and virus-like particles; they are further described in the literature to be ultrasensitive to cisplatin
Biosafety level:
2
Risk assessment:
As described in literature, the GH cells produce human teratocarcinoma derived viruses (HDTV) which are identical to human endogenous retrovirus K (HERV-K). HERV-K is assigned to biosafety level 2 in Germany. The cell culture supernatant shows a marginal reverse transcriptase activity in the cell culture supernatant which can slightly be stimulated by 5-azacytidine and strongly applying 5-iodo-2-deoxyuridine. The activity was determined employing the product enhanced reverse transcriptase assay (PERT)
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
polygonal cells growing adherently in monolayers and discrete colonies
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:2 - 1:4 once or twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
cell harvest of ca. 8 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV-, HERV-K +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list