KMST-6-TNF
ACC 824
Cell line:
KMST-6-TNF
DSMZ no.:
ACC 824
Species:
human (Homo sapiens)
Cell type:
fibroblasts
Origin:
Immortalized human fibroblasts established in 1982 by Gamma-irradiation of isolated fibroblasts from a 9-week old female fetus (KMST-6). Subsequent transformation with a plasmid containing human TNF-alpha cDNA
confering TNF production and hygromycin resistance
Biosafety level:
1, GMO-S1
Risk assessment:
The original cells (KMST-6) were transfected with a plasmid (pCDL-81) containing the cDNA for TNFalpha and selected via hygromycin resistence. Neither the cells nor TNFa present a danger for humans and thus the cell line is classified as S1
DSMZ Cell Culture Data:
Morphology:
adherent fibroblastoid cells growing as monolayer
Medium:
90% MEM (with Earle salts) + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells/80 cm2;
split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50 hours
Harvest:
ca. 4-5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
is described by Namba et al. to carry structural and numerical chromosomal aberrations
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list