JVE-528
ACC 826
Cell line:
JVE-528
DSMZ no.:
ACC 826
Species:
human (Homo sapiens)
Cell type:
colon carcinoma
Origin:
established from the primary tissue of the ascending colon of a 57-year-old woman with malignant adenocarcinoma (Dukes stage B1); cells were described in literature to carry mutations in the KRAS, TP53 and APC genes
and methylation of the MGMT promoter
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at ca. 2-3 x 106 cells/25 cm2;
split confluent culture 1:2 once a week using trypsin/EDTA; however cells may need several days to adhere after thawing and trypsination; we recommend to use always a new culture flask after trypsination
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 4 days
Harvest:
cell harvest of 12-14 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list