CHP-100
ACC 830
Cell line:
CHP-100
DSMZ no.:
ACC 830
Species:
human (Homo sapiens)
Cell type:
Ewing's sarcoma
Origin:
established from epidural tumor tissue of the spine obtained by thoracic laminectomy of a 12-year old girl with diagnosed neuroepithelioma. Cell line was reported in literature to be tumorigenic in nude mice and in a later analysis erroneously as neuroepithelioma. At DSMZ, it was shown that CHP-100 was misclassified because further analysis demonstrated the chromosomal translocation t(11;22) and the fusion gene product EWS/FLI
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
epithelioid cells growing adherently as monolayer; only very few cells exhibit a neuronal-like morphology
Medium:
90% McCoy's 5A + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2;
split confluent culture 1:3 to 1:4 every 3-4 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 36 hours
Harvest:
cell harvest of 10-15 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
expression of fusion gene EWS-FLI1 was confirmed by RT-PCR
Viruses:
PCR: HBV -, HCV -, EBV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list