EL-4
ACC 831
Cell line:
EL-4
DSMZ no.:
ACC 831
Species:
murine (mouse) (Mus musculus)
Cell type:
T cell lymphoma
Origin:
established from a Dimethyl-benzanthracene induced lymphoma in a C57BL/6N mouse
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
small, rounded to oval or multiform cells growing mostly singly in suspension; some cells exhibit tiny hairs or fringes
Medium:
95% RPMI 1640 + 5% h.i. FBS
Subculture:
seed out at ca. 0.3-0.5 x 106 cells/ml; maintain between 1.0 and 1.5 x 106 cells/ml; split saturated culture 1:3-1:5 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 15-20 hours
Harvest:
maximal density at about 1.8 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Viruses:
PCR: MLV +
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list