U2OS#18
ACC 832
Cell line:
U2OS#18
DSMZ no.:
ACC 832
Species:
human (Homo sapiens)
Cell type:
osteosarcoma
Origin:
Genetic modification of human osteosarcoma cell line U-2-OS for studying sister chromatid recombination (SCR). The cell line is described to contain a single copy of the SCR reporter inserted as nested intron and carries the GFP-Gene and is puromycin resistant. A series of modified cell lines with gene disruptions in RAD51 paralogs are also available (DSMZ ACC 833-ACC 842).
Biosafety level:
1, GMO-S1
Risk assessment:
genetically modified organism, according to German and European legislation allocated into group S1
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
90% DMEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine; (to verify puromycin resistance, 1 µg/ml should be added)
Subculture:
seed out at 1 x 106 cells/80 cm2;
split confluent culture once or twice a week 1:3 to 1:10 using trypsin/EDTA; apparently confluent cultures can be further cultivated for 2-3 days to increase the maximal cell harvest
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 50-60 hours
Harvest:
cell harvest of 5-10 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list