U2OS#18-RAD51D-4
ACC 835
Cell line:
U2OS#18-RAD51D-4
DSMZ no.:
ACC 835
Species:
human (Homo sapiens)
Cell type:
osteosarcoma
Origin:
Genetic modification of U2OS#18 (DSMZ ACC 832, a reporter cell line for measurement of sister chromatid recombination, SCR) with a genetic disruption of RAD51D by frameshifting mutations in exon 1 (targeted by
CRISPR-CAS9; puromycin resistant and expressing GFP). The wild-type allele is no longer present. A sister cell line with the retrovirally reconstituted RAD51D gene is available as ACC 840
Biosafety level:
1, GMO-S1
Risk assessment:
genetically modified organism, according to German and European legislation allocated into group S1
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
80% DMEM + 20% h.i. FBS + (additionally) 2 mM L-glutamine (to verify puromycin resistance, 1 µg/ml should be added)
Subculture:
seed out at 0.5-1 x 106 cells/80 cm2;
split confluent culture once a week 1:2 to 1:3 using trypsin/EDTA (wash twice with PBS before adding trypsin/EDTA); apparently confluent cultures can be further cultivated for 4-6 days to increase the maximal cell harvest
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 4-6 days
Harvest:
cell harvest of ca. 4-8 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
frameshift mutations in exon 1 of RAD51D were verified by sequence analysis
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list