SPI-801 - Discontinued
ACC 86
Cell line:
SPI-801 - Discontinued
DSMZ no.:
ACC 86
Species:
human (Homo sapiens)
Cell type:
chronic myeloid leukemia in blast crisis (cell line K-562)
Origin:
Caution: STR analysis demonstrated that cell line SPI-801 is genetically identical to human chronic myeloid leukemia celll line K-562. SPI-801 must not be used as model for human T-ALL.
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
round, large, single cells in suspension
Medium:
90-95% RPMI 1640 + 5-10% h.i. FBS
Subculture:
seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hrs
Harvest:
maximal density at about 1.2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays
Immunology:
CD3 -, CD19 -, CD71 +, CD235a +; image
Authenticity:
STR analysis resulted in the STR profile of cell line K-562.
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list