JK-6
ACC 860
Cell line:
JK-6
DSMZ no.:
ACC 860
Species:
human (Homo sapiens)
Cell type:
multiple myeloma
Origin:
established from peripheral blood of a 75-year-old male with multiple myeloma in 1992. Cells were originally IL-6 dependent but became independent during the further course of establishment. Described to carry mutations in the K- and N-RAS genes. More cytogenetic and immunological data, including HLA types are available upon request to mutz@dsmz.de
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
single round (plasmablasts-like) cells growing in suspension
Medium:
80% RPMI 1640 + 20% FBS + 2 mM L-glutamine
Subculture:
seed out at about 0.5 x 106 cells/ml; maintain at 0.5-1.0 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 48 hours
Harvest:
maximal density of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG +, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list