INA-6
ACC 862
Cell line:
INA-6
DSMZ no.:
ACC 862
Species:
human (Homo sapiens)
Cell type:
multiple myeloma
Origin:
established from the pleural effusion of an 80-year old patient with plasma cell leukemia in 1988. Cell line is IL-6 dependent and was described in the literature to carry an activating mutation of the N-RAS gene and del(1)(p22), del(7)(p13) and 14q+: t(11;14)(q13;q32) translocation
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% FBS + 10 ng/ml IL-6
Subculture:
seed out 0.5-1.0 x 106 cells/well in a 24-well plate or at 0.5 x 106 cells/ml in a small culture flask; maintain at 0.5-1.5 x 106 cells/ml, split 1:2 to 1:4 every 2-3 days. After thawing growth may be delayed
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-50 hours
Harvest:
maximal density of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 -, cyCD79a -, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, smkappa -, cykappa +, sm/cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list