UPCI-SCC-081

human (Homo sapiens)

oral squamous cell carcinoma

established in 1994 from a primary tumor tissue of a squamous cell carcinoma (stage T4N0) of the alveolar ridge of a 87-year-old woman. Described to carry a chromosomal 11q13 amplification and some additional alterations. The cell line will only be distributed to non-profit institutions, commercial entities may apply for a license from Maria Vanegas (msv4@pitt.edu / 001-414-648-4004) at the University of Pittsburgh, Office of Technology Management

14735, 15061, 18200

1

A

The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004).

small epithelioid cells growing adherently as monolayer; image; image

80% MEM (with Earle's salts) + 20% h.i. FBS + 2 mM L-glutamine

seed out at about 1-2 x 10⁶ cells/80 cm²; split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA (wash 2x with PBS and then incubate for up to 10 min with trypsin)

at 37 °C with 5% CO₂

ca. 48 hours

cell harvest of ca. 8-10 x 10⁶ cells/80 cm² flask

frozen with 70% medium, 20% FBS, 10% DMSO

contamination was eliminated with Plasmocin, then negative in PCR assay

to make inquiries about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -

Delivery form		Prices
Frozen culture		450,- €
Growing culture   (please inquire for exact delivery time)		900,- €
DNA isolated from cell line (25 µg)		560,- €
DNA isolated from cell line (5 µg)		140,- €

see price list

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