LB5848-LYMP
ACC 870
Cell line:
LB5848-LYMP
DSMZ no.:
ACC 870
Species:
human (Homo sapiens)
Cell type:
B cell lymphoma
Origin:
established in 2016 from a parotid lymph node of a 75-year-old man with diffuse large B-cell lymphoma (GCB subtype, stage IB). Cells are described to exhibit an abnormal hyperdiploid and complex karyotype including BCL2 and MYC translocations (double-hit DLBCL).
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
cells are difficult to culture; seed out at 0.25 x 106 cells in 2ml in a 24-well-plate (alternatively 0.1 x 106 cells/ml in a culture flask); maintain at 0.25-1.0 x 106 cells/ml (do not exceed 1.5 x 106 cells/ml); split 1:2 to 1:4 every 2-4 days; cell viability decreases with increasing cell density
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 50-70 hours
Harvest:
maximal density of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD79a (+), CD80 (+), CD138 -, HLA-DR -,
sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
expression of BCL2 and MYC confirmed by western blotting
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list