human (Homo sapiens)
This cell line is part of a set of cell lines carrying mutations in different genes affecting mRNA processing and translation efficiency (ACC 872 - ACC 876). Single clone derivative of 293T (DSMZ ACC 635) which was modified by CRISPR/CAS9 editing. In this cell line the ORFs of both alleles of the HELZ gene was disrupted by an insertion in exon 8. The HELZ (Helicase with Zinc-finger) protein is interacting with the CCR-NOT transcription complex promoting decapping of mRNAs and their decay.
genetically modified organism, according to German and European legislation allocated into group S1 (see also parental 293T, DSMZ ACC ACC 635)
DSMZ Cell Culture Data:
90% Dulbecco´s MEM + 10% h.i. FBS + 2mM L.-glutamine
seed out at about 0.5-1 x 106 cells/80cm2; split confluent culture 1:3 to 1:10 every 2-3 days by tapping the flask (trypsin/EDTA is not necessary)
at 37 °C with 5-10% CO2
ca. 24-30 hours
cell harvest of ca. 4-10 x 106 cells/80 cm2 flask
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in PCR assay
to inquire about expression of EpCAM and intermediate filaments, contact firstname.lastname@example.org.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
disruption of the HELZ gene was verified by sequence analysis as described in Ref 18207. For internal control the following primers can be used: D-HELZ-F: GGTGTTATGAAGAGGAGAGTAAAGTGA, D-HELZ-R: TGTACTAGCTTAGGACAGAGAGAGAAG. Absence of the HELZ protein was confirmed by Western blot.
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -
|Frozen culture||400,- €|
|Growing culture |
(please inquire for exact delivery time)
|DNA isolated from cell line (25 µg)||500,- €|
|DNA isolated from cell line (5 µg)||120,- €|
see price list