DLRP
ACC 881
Cell line:
DLRP
DSMZ no.:
ACC 881
Species:
human (Homo sapiens)
Cell type:
lung squamous cell carcinoma
Origin:
established in 1989 from a metastasis of a cervical lymph node of a 61-year-old male with a poorly differentiated squamous cell carcinoma of the lung
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
Transfer to third parties needs to be negotiated with the depositor.
DSMZ Cell Culture Data:
Medium:
DMEM + Ham's F12 (1:1) + 10-20% h.i. FBS
Subculture:
seed out at about 0.5 x 106 cells in a 25 cm2 flask; split 1:2 once a week using trypsin/EDTA; optimal cell density 1-2 x 106 cells/80 cm2; cells grow very slowly
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 1 week
Harvest:
cell harvest of ca. 3 x 106 cells/80 cm2 flask
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list