HCC-33BL
ACC 889
Cell line:
HCC-33BL
DSMZ no.:
ACC 889
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells
Origin:
lymphoblastoid cell line established in 1998 from peripheral blood of a 52-year-old man with small cell lung carcinoma; autologous carcinoma cell line HCC-33 available (DSMZ ACC 487)
Biosafety level:
2
Risk assessment:
The cell line carries human herpesvirus 4 sequences (HHV-4, Epstein-Barr virus, EBV) which were detected by PCR. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at about 1 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml, split 1:2 to 1:3 every 2-3 days; cell clumps may carefully be dispersed to encourage growth
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 48 hours
Harvest:
maximal density of 1.5-2.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37+, CD80 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database matching the autologous cell line HCC-33
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list