NCI-BL1184
ACC 896
Cell line:
NCI-BL1184
DSMZ no.:
ACC 896
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells
Origin:
lymphoblastoid cell line established in 1985 from peripheral blood of a 42-year-old man with metastatic small cell lung carcinoma; autologous carcinoma cell line H-1184 is available (DSMZ ACC 502)
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2.
DSMZ Cell Culture Data:
Morphology:
Medium:
80% RPMI 1640 + 20% h.i. FBS
Subculture:
seed out at about 0.5 x 106 cells/ml; maintain at 1- 2 x 106 cells/ml, split 1:5 to 1:10 once or twice a week
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 44 hours
Harvest:
maximal density of 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the autologous cell line H-1184
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1-, HIV-2 -, HTLV-1/2-, MLV-
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list