Species:
human (Homo sapiens)
Cell type:
multiple myeloma
Origin:
established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968; cells were described in the literature to produce IgE lambda; possible fusion partner for hybridoma production; cells express mRNA for BCL2 gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and
RNA-Seq)
Permissions and restrictions:
Morphology:
round to polygonal, single or clustered cells in suspension, some loosely adherent;
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Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Subculture:
seed out at ca. 0.5-0.7 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; maintain at 0.2-1.0 x 106 cells/ml; cells grow partially (loosely) adherent and can be removed with a bent Pasteur pipette (trypsin is not necessary)
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 55 hours
Harvest:
saturation density at 1.0-1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
Mycoplasma:
initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, and continuously in PCR assays
Immunology:
CD3-, CD10-, CD13-, CD19-, CD20-, CD34-, CD37-,
CD38-, CD80-, CD138+, HLA-DR(+), cyCD79a-,
cIgG-, cIgM-, cIg-kappa-, cIg-lambda+, sIgG-, sIgM-,
sIg-kappa-, sIg-lambda-;
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Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human complex hypodiploid karyotype with 6.5% polyploidy - 44(40-46)<2n>XY, -8, -10, -13, -15, +2mar, t(1;11)(p33;q13), add(3)(q27), t(4;11)(q?21;q23), add(7)(q32), add(8)(q24), add(9)(q34), add(10)(p14), add(14)(p11), add(17)(p11), add(18)(p12), der(22)t(15;22)(q21;q13) - 11q13 breakpoint recurrent in multiple myeloma
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | | Prices |
|
Growing culture
(please inquire for exact delivery time) | | 1040,- € |
| Frozen culture | | 520,- € |
| DNA isolated from cell line (25 µg) | | 650,- € |
| DNA isolated from cell line (5 µg) | | 160,- € |
see price list
