WERI-RB-1
ACC 90
Cell line:
WERI-RB-1
DSMZ no.:
ACC 90
Species:
human (Homo sapiens)
Cell type:
retinoblastoma
Origin:
established from retinoblastoma tissue of a 1-year-old girl in 1974; cells are described to initiate tumors in rabbits and lost completely the RB1 gene
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
optimal split ratio of 1:2 to 1:3 once a week; maintain at ca. 0.3-0.5 x 106 cells/ml; maximal cell density of about 1.5 x 106 cells/ml; seed out at ca. 0.3-0.5 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 49 hours
Harvest:
cell harvest of about 0.5-1.0 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS and 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human pseudodiploid karyotype with 3.9% polyploidy - 46(41-48)<2n>XX, +6, -10, -10, -14, -22, +3mar, add(3)(q25), add(3)(q25), add(4)(p15), add(5)(q35), i(6q), del(7)(p21), add(9)(q33), der(13)x2, add(16)(q23), add(16)(q23), i(17q), add(19)(q13) - apparently (uniparental?) disomic rearrangement of ch 13 - corresponds to reported karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list