NCI-H209
ACC 900
Cell line:
NCI-H209
DSMZ no.:
ACC 900
Species:
human (Homo sapiens)
Cell type:
small cell lung carcinoma
Origin:
established in 1979 from the bone marrow aspirated from a 55-year-old man with small cell lung carcinoma prior to chemotherapy treatment (also named H-209); described as expressing neuroendocrine biochemical markers, e.g. neuron-specific enolase, brain creatine kinase, L-DOPA decarboxylase and bombesin and to be tumorigenic in nude mice;
listed in the CCLE (ref 18232) with extended genome and protein analyses
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
80-90% RPMI 1640 + 10-20% h.i. FBS
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-80 hours
Harvest:
maximum density of ca. 2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
Sequencing confirmed a point mutation of the RB1 gene (codon 706: Cys -> Phe); a wild-typ allele was not detected
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list