A-431
ACC 91
Cell line:
A-431
DSMZ no.:
ACC 91
Species:
human (Homo sapiens)
Cell type:
epidermoid carcinoma
Origin:
established from the solid tumor of an 85-year-old woman; cells were reported to have large numbers of EGF binding sites; cell line was used as indicator cell line for anti-TGF binding
Reference(s):
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:4 to 1:6 every 4-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 80-100 hours
Harvest:
about 7-12 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid karyotype with 24% polyploidy - 64(58-64)<3n>XX/XXX, -2, +3, +7, -8, +10, -11, -13, -14, -15, -16, -18, -22, +2mar - del(3)(p11)x1-2, add(4)(p15), add(6)(p21.3), del(7)(p15), der(7)t(7;11)(p11/13;q11/13)add(11)(q23), add(8)(p21), add(11)(q23), der(14)t(13;14)(q11;p11), add(16)(p13) - carries t(7;11) with breakpoint at EGFR (which it overexpresses) - corresponds to published karyotype
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list