MBU-8
ACC 910
Cell line:
MBU-8
DSMZ no.:
ACC 910
Species:
human (Homo sapiens)
Cell type:
histiocytic lymphoma (subclone of U-937)
Origin:
subclone of U-937 developed of parental cell line from ATCC. Please note: U-937 available at DSMZ (ACC 5) was obtained from the establisher's lab from the University of Uppsala; sister cell line of MBU-7 (DSMZ ACC 909). MBU-8 is described to carry several cytogentic differences to U-937 and MBU-8; MBU-7 and MBU-8 differ in their sensitivity to azacitidine, decitabine and cytarabine
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at about 0.4 x 106 cells/ml; maintain at 0.5-1 x 106 cells/ml; split 1:3 to 1:5 every 3-4 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30 hours
Harvest:
maximal density of ca.2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD4 +, CD10 -, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD38 -, CD44 +, CD54 +, HLA-DR -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Molec. Genetics:
genomic profiling analysis (array-CGH) confirmed partial trisomy 7, tetrasomy 8(p) and trisomy 18
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list