BL-37
ACC 918
Cell line:
BL-37
DSMZ no.:
ACC 918
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1981 from the bone marrow of a 3-year old boy diagnosed with Burkitt lymphoma. The cells are EBV+ and are described to carry a t(8;22) translocation leading to MYC-IGL fusion; a matched B-lymphoblastoid cell line is available (IARC-176A, DSMZ ACC 926)
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Iscove's MDM + 10% h.i. FBS + 100 µM alpha-thioglycerol
Subculture:
seed out at 0.3-0.5 x 106 cells/ml; split 1:2 to 1:3 every 2-3 days and maintain at 0.3-1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 50-60 hours
Harvest:
maximal density of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD30 +, CD34 -, CD37 +, CD38 +, CD80 +, CD138 -, HLA-DR +, cy IgM (+), cy IgG -, cy kappa +, cy lambda -
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Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching autologous IARC-176A
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list