BL-64
ACC 919
Cell line:
BL-64
DSMZ no.:
ACC 919
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established in 1982 from the bone marrow of a 4-year-old boy with Burkitt lymphoma in relapse; cells are EBV-positive and were described to carry a t(2;8) translocation leading to MYC-IgK fusion; a matched B-lymphoblastoid cell line is available (IARC-549, DSMZ ACC 927)
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in phorbol ester / sodium butyrate stimulated cells. The infection was classified as potentially lytic associated with a production of active viruses. A transmission of EBV during handling of the cells cannot be excluded and the cell line is therefore classified biosafety level 2.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Iscove's MDM + 10% h.i. FBS + 100 µM Thioglycerol
Subculture:
seed out at about 0.3 x 106 cells/ml; split
1:3 to 1:10 every 2-4 days; maintain at 0.2 x 106 - 0.5 x 10 6 cells/m
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 30-50 hours, depending on cell density
Harvest:
maximal density of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD30 -, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, cyIgM +, cyIgG -, cykappa +, cylambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching autologous IARC-549
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
Delivery form | Prices | |
Frozen culture | 450,- € | |
Growing culture (please inquire for exact delivery time) | 900,- € | |
DNA isolated from cell line (25 µg) | 560,- € | |
DNA isolated from cell line (5 µg) | 140,- € |
see price list