BL-67
ACC 921
Cell line:
BL-67
DSMZ no.:
ACC 921
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from ascites fluid of a 35-year old female with EBV-positive Burkitt lymphoma; desribed to carry t(14;18) chromosomal translocation, resulting in IgH-MYC fusion transcripts
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was detected by western blot after stimulating the cells with phorbol ester / sodium butyrate. The infection was classified as potentially lytic associated with a possible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Iscove's MDM + 10% h.i. FBS + 100 µM Thioglycerol
Subculture:
seed out at 0.1 - 0.5 x 106 cells/ml; split
1:4 to 1:10 every 2-5 days; optimal density 0.3 - 1.0 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 10-25 hours
Harvest:
maximal density of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cy IgM +, sm/cykappa -, sm/cy lambda +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list