BL-74
ACC 922
Cell line:
BL-74
DSMZ no.:
ACC 922
Species:
human (Homo sapiens)
Cell type:
Burkitt lymphoma
Origin:
established from the ascites of a 33-year old woman with sporadic Burkitt lymphoma after relapse; cells are EBV+ and described to carry a t(8;14) translocation leading to MYC-IGH fusion, a third chromosome 10 and a homozygous deletion at 6q26-q27 (D6S347); a matched B-lymphoblastoid cell line is available (IARC-290A, DSMZ ACC 929)
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2.
DSMZ Cell Culture Data:
Morphology:
mostly round cells growing singly and partly in small clusters in suspension; image
Medium:
80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at 0.3-0.5 x 106 cells/ml; split 1:3 to 1:4 every 2-3 days and maintain at 0.5-1 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 45 hours
Harvest:
ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD30 +, CD34 -, CD38 +, CD80 +, CD138 -, HLA-DR +, cy kappa +, cy lambda -; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, , matching autologous IARC-290A
Viruses:
PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list