CAL-1
ACC 923
Cell line:
CAL-1
DSMZ no.:
ACC 923
Species:
human (Homo sapiens)
Cell type:
plasmacytoid dendritic cell line
Origin:
established in 2000 from the peripheral blood of a 76-year-old man with blastic natural killer cell lymphoma in leukemic phase after chemotherapy.
Cells are positive for CD4 and CD56 and were classified as plasmacytoid dendritic cells (pDC) in the original paper
Reference(s):
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
cell line will only be distributed to academia; transfer to third parties needs to be negotiated with the depositor; patented cell line, which is deposited as FERM P-20595 in Japan. International PCT application as FERM BP-10914.
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS + 2mM L-glutamine
Subculture:
seed out at about 0.5 x 106 cells/ml; split
1:2 to 1:4 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 25-35 hours
Harvest:
maximal density of ca. 1.5 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with BM-Cyclin,
then negative in PCR assays
Immunology:
CD3 -, CD4 +, CD5 -, CD6 -, CD7 -, CD8 -, CD13 -, CD14 -, CD19 -, CD33 +, CD34 -, CD38 +, CD56 +, CD71 +, CD80 -, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list