IARC-176A
ACC 926
Cell line:
IARC-176A
DSMZ no.:
ACC 926
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells
Origin:
B95-8-EBV-transformed cell line established in 1981 from normal B-cells of peripheral blood of a 3-year-old boy with Burkitt lymphoma; an autologous BL cell line is available (BL-37, DSMZ ACC 918)
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2.
DSMZ Cell Culture Data:
Morphology:
Medium:
90% Iscove's MDM + 10% h.i. FBS + 100 µM alpha-thioglycerol
Subculture:
seed out at about 0.5 x 106 cells/ml; split 1:3 to 1:4 every 2-3 days, maintain at 0.2-0.4 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 25-30hrs
Harvest:
maximal density of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD80 +, HLA-DR +; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the autologous cell line BL-37
Viruses:
PCR: EBV + (cells carry the EBV strain B95.8), HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list