IARC-305
ACC 928
Cell line:
IARC-305
DSMZ no.:
ACC 928
Species:
human (Homo sapiens)
Cell type:
B lymphoblastoid cells
Origin:
B95-8-EBV-transformed cell line established in 1983 from normal B-cells of peripheral blood of a 3-year-old girl with Burkitt lymphoma; an autologous Burkitt lymphoma cell line is available (BL-65, DSMZ ACC 920)
Reference(s):
Biosafety level:
2
Risk assessment:
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot analysis in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is therefore classified biosafety level 2.
DSMZ Cell Culture Data:
Medium:
80-90% Iscove's MDM + 10-20% h.i. FBS + 2 mM L-glutamine + 100 µM alpha-thioglycerol
Subculture:
seed out at about 0.5 x 106 cells/ml; split 1:2 to 1:3 every 3-5 days, maintain at 0.3-0.8 x 106 cells/ml
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 2-4 days
Harvest:
maximal density of ca. 1 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Immunology:
CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +, cyIgG +, cyIgM - ; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, matching the autologous cell line BL-65
Viruses:
PCR: EBV + (cells carry the EBV strain B95-8), HBV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list