HEP-3B
ACC 93
Cell line:
HEP-3B
DSMZ no.:
ACC 93
Species:
human (Homo sapiens)
Cell type:
hepatocellular carcinoma
Origin:
established from the tumor tissue of an 8-year boy in 1976; cells contain a 2.3 kb integrated hepatitis B virus genome fragment; cells were described in the literature to produce a variety of proteins, e.g. alpha-fetoprotein, albumin, transferrin, alpha2-macroglobulin, alpha1-antitrypsin, haptoglobin and others
Biosafety level:
1
Risk assessment:
the cell line contains parts of the hepatitis B virus (HBV) genome integrated into the eukaryotic genome. The HBV surface antigen (HBsAg) is expressed, but no active viruses are produced by the cells. The cells can thus be handled under biosafety level 1. According to the German Central Committee for Biological Safety (ZKBS) the cells can also be handled under biosafety level 1 when the cells are used for genetic modifications.
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine
Subculture:
seed out at ca. 0.5-1 x 106 cells/80 cm2;
split confluent culture 1:4 to 1:10 every 3-5 days using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
about 40-50 hours
Harvest:
about 5 x 106 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de.
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
Cytogenetics:
human hypotriploid stemline with near tetraploid side line (6%) - 63 (60-64)<3n>XX, -Y, -1, -1, -2, -3, -4, -8, -9, -13, +der(11)t(1;11)(q11;q14)x2, der(?15)t(1;?15)(q11;q24), i(7p)
Viruses:
ELISA: reverse transcriptase negative; PCR: EBV -, HBV + (see risk assessment), HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list