NS20Y
ACC 94
Cell line:
NS20Y
DSMZ no.:
ACC 94
Species:
murine (mouse) (Mus musculus)
Cell type:
neuroblastoma
Origin:
established from a A/J mouse strain with neuroblastoma C1300; cells were described in the literature to be aneuploid and to contain high specific activity of choline acetyltransferase
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Morphology:
Medium:
90% DMEM + 10% h.i. FBS
Subculture:
split semi-confluent culture 1:5 to 1:10 using trypsin/EDTA (cells can also be detached by tapping the flask); seed out at ca. 2 x 106 cells/80 cm2; viability might decrease to 50-70%; maximal density at ca. 5-10 x 106 cells/80 cm2
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 30-50 hours
Harvest:
cell harvest of about 1 x 107 cells/80 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Species validation:
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species.
Cytogenetics:
murine karyotype
Viruses:
ELISA: reverse transcriptase positive; PCR: SMRV -
Supplied as:
| Delivery form | Prices | |
| Frozen culture | 450,- € | |
| Growing culture (please inquire for exact delivery time) | 900,- € | |
| DNA isolated from cell line (25 µg) | 560,- € | |
| DNA isolated from cell line (5 µg) | 140,- € | |
see price list