Leibniz Institute DSMZ

Cell line:

CMS

DSMZ no.:

ACC 985

Species:

human (Homo sapiens)

Cell type:

acute megakaryocytic leukemia

Origin:

established in 1991 from a 2-year-old girl with pediatric acute myeloid leukemia (AML M7) at relapse

Reference(s):

Biosafety level:

1

Permissions and restrictions:

Depositor-specific restrictions:

The cell line will only be distributed to non-profit institutions.

DSMZ Cell Culture Data:

Morphology:

round cells growing singly or in small clumps in suspension; image; image; image

Medium:

80-90% RPMI 1640 + 10-20% h.i. FBS

Subculture:

seed out at about 0.5 x 10⁶ cells/ml and maintain later between 0.3 and 0.5 x 10⁶ cells/ml, split 1:2 to 1:3 every 2-3 days

Incubation:

at 37 °C with 5% CO₂

Doubling time:

ca. 48 hours

Harvest:

maximal density of 1.0 x 10⁶ cells/ml

Storage:

frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:

Mycoplasma:

negative in PCR assay

Immunology:

CD3-, CD4-, CD13+, CD14-, CD15+, CD19-, CD33+, CD34+, HLA-DR+; image

Authenticity:

STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database

Viruses:

PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -

Supplied as:

Delivery form		Prices

Growing culture (please inquire for exact delivery time)		1040,- €
Frozen culture		520,- €
DNA isolated from cell line (25 µg)		650,- €
DNA isolated from cell line (5 µg)		160,- €