CTS
ACC 987
Cell line:
CTS
DSMZ no.:
ACC 987
Species:
human (Homo sapiens)
Cell type:
acute megakaryocytic leukemia
Origin:
established in 1982 from peripheral blood of a 13-year-old girl with poorly differentiated megakaryocytic leukemia (AML M1) at relapse after autologous BMT
Biosafety level:
1
Permissions and restrictions:
Depositor-specific restrictions:
The cell line will only be distributed to non-profit institutions.
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out 0.5-1.0 x 106 cells/ml and maintain between 0.5 and 1.5 x 106 cells/ml, split 1:2 to 1:4 every 2-3 days
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 48 hours
Harvest:
maximal density of 1.5-2 x 106 cells/ml
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays
Immunology:
CD3-, CD4-, CD7+, CD13+, CD14-, CD15+, CD19-,
CD33+, CD34+, HLA-DR+, cyCD3-, cyCD68; image
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Molec. Genetics:
expression of fusion gene KMT2A::AFDN (alias MLL::AF6) confirmed by RT-PCR
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list