HELA-SLC35A1-KO
ACC 989
Cell line:
HELA-SLC35A1-KO
DSMZ no.:
ACC 989
Species:
human (Homo sapiens)
Cell type:
cervix carcinoma
Origin:
derivative of HELA, established in 2021 by CRISPR/Cas9-mediated modification of the CMP-sialic acid transporter-encoding gene SLC35A1 resulting in decreased/absent levels of the protein. The cells are described to harbour a functional knockout of the SLC35A1 gene leading to increased susceptibility to transduction with adeno-associated virus 9. The cells are therefore suited for AAV9 transduction potency assays
Reference(s):
Biosafety level:
1, GMO-S1
Risk assessment:
The parental cell line HELA is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cells are classified biosafety level 1 and the genetic modification renders the cell line level S1 according to German genetic engineering act.
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% DMEM (high glucose) + 10% h.i. FBS
Subculture:
seed out at ca. 0.5-1 x 106 cells in a T75 flask; split confluent culture 1:10 to 1:20 every 2-4 days using trypsin/EDTA
Incubation:
at 37 °C with 10% CO2
Doubling time:
ca. 15-20 hours
Harvest:
cell harvest of ca. 15 x 106 cells/T75 flask
Storage:
frozen with 70% DMEM, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ASN-0002.1 (2021) resulted in a STR profile identical to HELA (ACC 57) of the reference database.
Species validation:
human
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV+, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list