ICC6
ACC 997
Cell line:
ICC6
DSMZ no.:
ACC 997
Species:
human (Homo sapiens)
Cell type:
biliary tract cancer
Origin:
established in 2016 from a patient derived xenograft model (PDX) of the primary tumor of a 57-year-old woman suffering from intrahepatic cholangiocarcinoma. Cells are IDH1 wt and carry a mutation in TP53 p.K132T. Alias name: MGH-ICC6
Biosafety level:
1
Permissions and restrictions:
DSMZ Cell Culture Data:
Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out at ca. 1 x 106 cells in a T75 flask; split 1:2-1:4 once a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 3 days
Harvest:
cell harvest of ca. 10 x 106 cells/T175 flask
Storage:
frozen with 70% RPMI 1640, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in PCR assay
Authenticity:
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database
Species validation:
human
Molec. Genetics:
IDH1 wt and mutation of TP53 p.K132T were verified by RNA-seq analysis; positive for telomerase activity (by TRAP assay)
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -
Supplied as:
| Delivery form | Prices | |
| Growing culture (please inquire for exact delivery time) | 1040,- € | |
| Frozen culture | 520,- € | |
| DNA isolated from cell line (25 µg) | 650,- € | |
| DNA isolated from cell line (5 µg) | 160,- € | |
see price list