Cultivation conditions:
Medium 693 , microaerophilic with hydrogen, 37°C
(24% N
2, 6% O
2, 6% CO
2, 64% H
2). Cover the agar surface with enough (1 - 4 ml) liquid medium 215 to maintain a liquid film during the whole incubation period. Culture does not grow on dry surfaces. For reactivation distribute the complete content of the ampoule on one agar plate.
Incubation in a candle jar or in a jar equipped with commercially available microaerobic systems is not suited for the reactivation of freeze dried cellsIncubation time: 1-2 days
Please follow special instructions: 'Cultivation of Helicobacter'
Complete DSMZ Media List
Summary and
additional information:
<- NCTC <- C. S. Goodwin. [RPH 13491]. Human gastric antrum; Australia. Genetic map (6265). (Medium 693, 37°C, microaerophilic with hydrogen). (24% N2, 6% O2, 6% CO2, 64% H2). Cover the agar surface with enough (1 - 4 ml) liquid medium 215 to maintain a liquid film during the whole incubation period. Culture does not grow on dry surfaces. For reactivation distribute the complete content of the ampoule on one agar plate. Incubation in a candle jar or in a jar equipped with commercially available microaerobic systems is not suited for the reactivation of freeze dried cells.
