Name:
"Polynucleobacter brandtiae" Hahn et al.
DSM No.:
103491, Type strain dsm-103491 dsm103491 dsm 103491 Polynucleobacter brandtiae
Strain designation:
UB-Domo-W1
Other collection no.
or WDCM no.:
CIP 111598,JCM 32562
Country:
Antarctica
Byers Peninsula, Livingston Island
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- M. Hahn; <- Ulrike Brandt (Koll), University of Innsbruck, Research Institute for Limnology, Austria; UB-Domo-W1
Genbank accession numbers:
Cultivation conditions:
Medium 830 , 15°C
Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. They grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.
Complete DSMZ Media List
Summary and
additional information:
<- M. Hahn; <- Ulrike Brandt (Koll), University of Innsbruck, Research Institute for Limnology, Austria; UB-Domo-W1. Lake Domo. Antarctica, Byers Peninsula, Livingston Island. Type strain.. Taxonomy/description (40442). Sequence accession no. draft genome shotgun sequence: PGTX00000000, 16S rRNA gene: MK926443. (Medium 830, incubation time: more than 14 days, 15°C, aerobic). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. They grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.
Image:
cells of DSM 103491 on medium 830 at 15 °C
Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
Risk group:
1 (provisional classification by DSMZ)
Supplied as:
| Delivery form | | Prices |
|
| actively growing culture | | 160,- € | |
| DNA | | 150,- € | |
| Price Category for this culture: 1 |
Freight and handling charges will be added.
See price list.
