Nitrosopumilus piranensis

DSM 106147

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Name:

Nitrosopumilus piranensis Bayer et al. 2019

DSM No.:

106147, Type strain dsm-106147 dsm106147 dsm 106147 Nitrosopumilus piranensis

Strain designation:

D3C

Other collection no.
or WDCM no.:

JCM 32271,NCIMB 15115

Isolated from:

coastal surface water

Country:

Slovenia
Northern Adriatic Sea

Nagoya Protocol Restrictions:

There are NO known Nagoya Protocol restrictions for this strain.

History:

<- C. Sedlazek and B. Bayer, University Vienna, Fac. of Life Scs, Vienna, Austria; {2022} <- B. Bayer; {2017} <- B. Bayer, University Vienna, Fac. of Life Scs, Vienna, Austria; D3C

Genbank accession numbers:

complete genome: CP010868.1

Cultivation conditions:

Medium 1630a , 30°C
Medium/media recipe(s) required for preparing 1630a:
- Medium 141
- Medium 722

Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775). The delivery time is several months

Incubation time: more than 14 days

Complete DSMZ Media List

Summary and
additional information:

<- B. Bayer, Univ. Vienna, Austria; D3C. Coastal surface water; Slovenia, Northern Adriatic Sea. Type strain. Taxonomy/description (32586, 32605). Sequence accession no. complete genome: CP010868.1. Strain-specific literature (26775). (Medium 1630a, 30°C). Growth takes place without visible increase in turbidity, hence proliferation of cells has to be checked using phase contrast microscopy. Nitrite production is a reliable indication of growth and can be checked with Griess-Ilosvay's reagent. However, it is more accurate to measure consumption of ammounium using the OPA reagent as described by Holmes et al. 1999 (26775). The delivery time is several months.

Literature:

32605, 26775, 32586

Image: