Isolated from:
Petshop mouse, f, ca. 6w, small intestine
Summary and
additional information:
<- T. Clavel, RWTH Aachen; 0090-6-DD-A. Petshop mouse, f, ca. 6w, small intestine. Germany, Freising. (Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). , incubation time: 1-2 days, gas phase: 80%N2, 20%CO2, pH 7, 37°C, anaerobic or Medium 693, incubation time: 2-3 days, gas pack, pH 7, 37°C, anaerobic)
