Isolated from:
caecal content; SPF, female, 10-week-old, C57BL/6J mouse
Summary and
additional information:
<- T. Clavel, RWTH Aachen; CLA-AV-15. Caecal content; SPF, female, 10-week-old, C57BL/6J mouse. Germany, Aachen. Sequence accession no. 16S rRNA gene: OM658590. (Medium 339 Instead of Na-resazurin add 0,002 ml/ml Phenosafranin (Stock solution 1,25 mg/ml in H2O). After heating add 0,010 ml/ml Cystein-DTT (DL-Dithiothreitol)-solution (0,05 g/ml Cystein, 0,02 g/ml DTT). , incubation time: 1-2 days, gas phase: 80%N2, 20%CO2, pH 7, 37°C, anaerobic)
