Name:
Clostridioides difficile (Hall and O'Toole 1935) Lawson et al. 2016
DSM No.:
1296, Type strain dsm-1296 dsm1296 dsm 1296 Clostridioides difficile
Strain designation:
1780, McClung 1780, USDA 90556-M6S
Other collection no.
or WDCM no.:
ATCC 9689,NCIB 10666,NCTC 11209
Country:
country of origin unknown
Date of sampling:
before 24.04.1978
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- ATCC <- F.E. Clark, USDA 90556-M6S
Genbank accession numbers:
Cultivation conditions:
Medium 78 , anaerobic, 37°C
or
Medium 1203 , anaerobic, 37°C
strain grows very poorly on blood agar plates. For reactivation from ampoule cultivation in liquid media is strongly recommended! If you have to use solid medium, use M 1203 and inoculate one plate with the complete ampoule
Incubation time: 1-2 days
Please follow special instructions: 'Cultivation of Anaerobes'
Complete DSMZ Media List
Summary and
additional information:
<- ATCC <- F.E. Clark, USDA 90556-M6S. country of origin unknown; sampling date: before 24.04.1978. Type strain. Taxonomy/description (1300, 20760, 20782, 26004, 26018). Sequence accession no. 16S rRNA gene: AB075770, 16S rRNA gene: NR_112172. Produces cytotoxin (see ATCC catalogue), but stated to be non-toxigenic (see NCTC catalogue) (5306). Produces cytotoxin (see ATCC catalogue), but stated to be non-toxigenic (see NCTC catalogue) (5307). Metabolism of amino acids (2197). Quality control strain according to DIN 58959-7 and 58959-9 (16730), Quality control strain according to DIN 58959-7 and 58959-9 (6924). Cellular lipid fatty acids (2199). Positive PCR for toxins A and B (tcdA and tcdB) and negative PCR for the binary toxin (ctdA and ctdB). Strain-specific literature (1934). (Medium 78, 37°C, anaerobic or Medium 1203, 37°C, anaerobic). Strain grows very poorly on blood agar plates. For reactivation from ampoule cultivation in liquid media is strongly recommended! If you have to use solid medium, use M 1203 and inoculate one plate with the complete ampoule.
Literature:
20782,
1934,
2197,
2199,
5306,
5307,
6924,
16730,
20760,
1300,
26004,
26018
Risk group:
2 (classification according to German
TRBA)
Restrictions:
Act dealing with the prevention & control of infectious diseases in man (Infektionsschutzgesetz),
Category A1
Supplied as:
| Delivery form | | Prices |
|
| Freeze Dried | | 100,- € |
| Active culture on request | | 240,- € |
| DNA | | 150,- € | |
| Price Category for this culture: 1 |
Freight and handling charges will be added.
See price list.Note: Freight charges for Risk Group 2 microorganisms will apply.
