Cupriavidus necator

DSM 13439

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How to read the following data (Example)

Name:

Cupriavidus necator Makkar and Casida 1987

DSM No.:

13439 dsm-13439 dsm13439 dsm 13439 Cupriavidus necator

Strain designation:

H850-4LT

Isolated from:

strain H850 (NRRL B-15940) genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase, xylXYZ of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway.

Country:

country of origin unknown
H850 was isolated from Hudson River dredge, USA

Date of sampling:

before 12.04.2000

Nagoya Protocol Restrictions:

There are NO known Nagoya Protocol restrictions for this strain.

History:

<- R M. Wittich, GBF, genetic modification <- NRRL

Cultivation conditions:

Medium 462 with 0.4 g/L 2-chlorobenzoate and 0.4 g/L 3-chlorobenzoate, 28°C
or
Medium 1a , 28°C
Medium/media recipe(s) required for preparing 1a:
- Medium 1

Complete DSMZ Media List

Summary and
additional information:

<- R M. Wittich, GBF, genetic modification <- NRRL. (Wautersia eutropha).Strain H850 (NRRL B-15940) was genetically modified by introduction of the genes cbdABC of a 2-halobenzoate dioxygenase xylXYZ, of a toluate dioxygenase and tcbRCDEF of a regulated chlorocatechol pathway.; H850 was isolated from Hudson River dredge soil, USA. GMO of H850 (NRRL B-15940). Utilizes monochlorinated benzoates, 3,5-dichlorobenzoate and polychlorinated biphenyls (PCBs) such as Arochlor 1221 or 1232 (13435). (Medium 462 with 0.4 g/L 2-chlorobenzoate and 0.4 g/L 3-chlorobenzoate, 20-30°C or Medium 1a, 20-30°C).

Literature:

13435

Risk group:

1 (classification according to German TRBA)

Restrictions:

German law regulating genetic engineering / Gentechnikgesetz), Category C

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