DSM No.:
15371 dsm-15371 dsm15371 dsm 15371 pSK (BEC/PP:3,3)
Remarks:
Genomic DNA (a 2.4 kb and a 0.9 kb PstI fragment) of Ralstonia eutropha HF39 was cloned into the 6.295 kb vector pSK(BEC/pp:3,3) which is a derivative of pBluescript SK- and transformed into the host E. coli XL1-Blue. On complex medium the cells produce a blue pigment characterised as indigo by several methods. The 1.251 kb ORF (bec) was cloned and sequenced. The gene product catalyzed formation of indoxyl, a reactive preliminary stage for indigo production. Strain forms long cells and colonies get an indigo blue colour after prolonged incubation.
Distributed in:
Escherichia coli SK 7863 (XL1-Blue derivative) tet, thi
Risk group:
1 (classification according to German
TRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),
Category C
Country:
country of origin unknown
Date of sampling:
before 19.12.2002
Nagoya Protocol Restrictions:
There are NO known Nagoya protocol restrictions for this strain.
