Name:
Polynucleobacter rarus Hahn et al. 2011
DSM No.:
21648, Type strain dsm-21648 dsm21648 dsm 21648 Polynucleobacter rarus
Strain designation:
MT-CBb6A5
Other collection no.
or WDCM no.:
CIP 109928
Isolated from:
water from the pelagic zone of an acidic lake
Country:
United States of America
Crystal Bog Lake, Wisconsin
Date of sampling:
31.05.2005
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-MT-CBb6A5 <- M. Tarao
Genbank accession numbers:
Cultivation conditions:
Medium 830 , slowly growing, keep agar surface moist, 28°C
Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.
Incubation time: 8-14 days
Complete DSMZ Media List
Summary and
additional information:
<- M. W. Hahn, Austrian Academy of Sciences, Inst. Limnology; MWH-MT-CBb6A5 <- M. Tarao. Water from the pelagic zone of an acidic lake; USA, Crystal Bog Lake, Wisconsin. Type strain. Taxonomy/description (15907). Sequence accession no. 16S rRNA gene: FM208182. (Medium 830, 28°C). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate..
Risk group:
1 (classification according to German
TRBA)
Supplied as:
| Delivery form | | Prices |
|
| actively growing culture | | 160,- € | |
| DNA | | 150,- € | |
| Price Category for this culture: 1 |
Freight and handling charges will be added.
See price list.
