Polynucleobacter aenigmaticus
DSM 24006
Name:
Polynucleobacter aenigmaticus Hahn et al. 2017
DSM No.:
24006, Type strain dsm-24006 dsm24006 dsm 24006 Polynucleobacter aenigmaticus
Strain designation:
MWH-K35W1, LMG 29706
Other collection no.
or WDCM no.:
or WDCM no.:
LMG 29706
Isolated from:
permanently anoxic water layer of a meromictic lake (monimolimnion)
Country:
Austria
Lake Krottensee, near St. Gilgen
Lake Krottensee, near St. Gilgen
Date of sampling:
22.09.2003
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- M. W. Hahn, Austrian Academy of Sci., Institute of Limnology, Mondsee; MWH-K35W1
Genbank accession numbers:
Cultivation conditions:
Medium 830 , 28°C
Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate
Complete DSMZ Media List
Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate
Complete DSMZ Media List
Summary and
additional information:
additional information:
<- M. W. Hahn, Austrian Academy of Sci., Institute of Limnology, Mondsee; MWH-K35W1. Permanently anoxic water layer of a meromictic lake (monimolimnion); Austria, Lake Krottensee, near St. Gilgen; sampling date: 22.09.2003. Type strain. Taxonomy/description (27526). Sequence accession no. 16S rRNA gene: CBI30_02030, whole genome shotgun sequence: NGUO00000000. (Medium 830, 28°C). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.
Literature:
Risk group:
1 (classification according to German TRBA)
Supplied as:
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DNA | 150,- € | ||
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