Name:
Polynucleobacter wuianus Hahn et al. 2017
DSM No.:
24008, Type strain dsm-24008 dsm24008 dsm 24008 Polynucleobacter wuianus
Strain designation:
QLW-P1FAT50C-4, CIP 111100
Other collection no.
or WDCM no.:
CIP 111100
Isolated from:
shallow acidic freshwater pond
Country:
Austria
Alps, Pond-1 (47° 44' 23.37'' N 13° 18' 6.03'' E) 1300 meters above sea level
Date of sampling:
Oct. 2003
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- M. W. Hahn, Austrian Academy of Science, Inst. Limnology, Mondsee; QLW-P1FAT50C-4 <- Q. L. Wu, Austrian Academy of Sci., Inst. Limnology
Genbank accession numbers:
Cultivation conditions:
Medium 830 , 22°C
or
Medium 1167 , 22°C
Medium/media recipe(s) required for preparing 1167:-
Medium 14-
Medium 27Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate.
Complete DSMZ Media List
Summary and
additional information:
<- M. W. Hahn, Austrian Academy of Science, Inst. Limnology, Mondsee; QLW-P1FAT50C-4 <- Q. L. Wu, Austrian Academy of Sci., Inst. Limnology. Shallow acidic freshwater pond; Austria, Alps, Pond-1 (47° 44' 23.37'' N 13° 18' 6.03'' E) 1300 meters above sea level (10512, 26603). Type strain. Taxonomy/description (13477, 25987, 26603). Sequence accession no. complete genome: CP015922, 16S rRNA gene plus ITS1: AJ879778. (Medium 830, 22°C or Medium 1167, 22°C). Polynucleobacter strains grow so weakly that single colonies or a streak of colonies might not be visible on the agar surface. These bacteria grow reliably only if the agar surface is moist. We grow the cultures generally on agar slants with 1.5 -2 ml liquid media added. The slants are inoculated and incubated upright for about 1 week. The bacteria will grow and settle down as a faint white precipitate at the ground of the slant (deepest point of liquid). That is how the cultures are prepared for delivery also. Transfer about 250 µl of the suspension to inoculate fresh slants. Growth will be indicated by the precipitate..
Image:
© Leibniz-Institut DSMZ
Risk group:
1 (classification according to German
TRBA)
Supplied as:
| Delivery form | | Prices |
|
| actively growing culture | | 160,- € | |
| DNA | | 150,- € | |
| Price Category for this culture: 1 |
Freight and handling charges will be added.
See price list.
